β-Actin—an unsuitable internal control for RT-PCR
نویسندگان
چکیده
منابع مشابه
Internal control for quality assurance of diagnostic RT-PCR.
Reverse transcription polymerase chain reaction (RT-PCR) is gaining major importance in the field of medical diagnostics. For example, RT-PCR assays are being tested to become standard methodology for detection of occult tumor cells in peripheral blood of cancer patients. However, there is heterogeneity in the frequency of tumor cell detection, e.g., in melanoma patients as reported in the lite...
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One approach to quantitate RNA is the use of a known number of competitive internal standard molecules that are reverse-transcribed and subsequently co-amplified under the same reaction conditions as the target sequence (3). This technique, called competitive reverse transcription polymerase chain reaction (cRT-PCR) has been applied to monitor the level of hepatitis C virus (HCV) RNA in patient...
متن کاملمعرفی و کلون سازی یک قطعه dna به عنوان یک کنترل داخلی (internal control) جهت تست rt-pcr رقابتی
نوویروس ها (nov) یکی از شایع ترین عوامل ایجاد کننده عفونت های غیرباکتریائی گاستروانتریت مزمن در جوامع انسانی می باشند. این ویروس ها اولین بار در سال1968 از اولین شیوع مربوط به مدرسه ای در منطقهnorwalk ایالت ohio آمریکا شناسائی گردیدند. نورویروس ها بسیار واگیردار بوده و به راحتی از طریق مدفوعی دهانی از فردی به فرد دیگر منتقل می شوند. بیماری، همراه با اسهال استفراغ بوده و 24-72 ادامه می یابد. این...
15 صفحه اولRelative quantitative RT-PCR protocol for TrkB expression in neuroblastoma using GAPD as an internal control.
An RT-PCR protocol for the relative quantitative measurement of TrkB transcripts using glyceraldehyde-3-phosphate-dehydrogenase (GAPD) transcripts as an internal control is described. Both TrkB and GAPD sequences were co-amplified in the exponential phase of amplification using 5'-biotinylated primers. The PCR products were subjected to PAGE, electro-transferred to nylon membrane and detected b...
متن کاملConstruction of an internal control to quantitate multiple porcine cytokine mRNAs by RT-PCR.
A multiple internal control was constructed to be used as an exogenously added control in reverse transcription polymerase chain reaction (RT-PCR) for pig cytokines. It consists of 5' and 3' primer sequences in the order of beta 2 microglobulin (beta 2-m), IL-1, IL-4, IL-6, IL-8, IL-2, IL-10, TNF-alpha, TNF-beta and IFN-gamma. Construction was accomplished by overlapping and extension PCR (OE-P...
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ژورنال
عنوان ژورنال: Molecular and Cellular Probes
سال: 2001
ISSN: 0890-8508
DOI: 10.1006/mcpr.2001.0376